山东省局部地区番茄黄化曲叶病毒的分子鉴定Molecular Identification of A New Tomato Yellow Leaf Curl Virus in Partial Regions of Shandong Province
褚栋;侯丽霞;刘国霞;高长生;Judith K Brown;
摘要(Abstract)：
近年来山东省部分地区发现番茄黄化曲叶症状。为了确定这种疑似病毒,利用双生病毒外壳蛋白(CP)基因及DNA-A基因组的引物对枣庄、菏泽和淄博的染病番茄叶片进行了检测,测序分析表明这种病毒是番茄黄化曲叶病毒(TYLCV)。系统发育分析结果表明,该病毒可能是由我国东南沿海地区和日本传入的。
关键词(KeyWords)： 番茄黄化曲叶病毒;山东省;PCR
基金项目(Foundation): 国家科技支撑计划重大项目(2006BAD08A18);; 农业部公益性行业科研专项项目(200803005)
作者(Author): 褚栋;侯丽霞;刘国霞;高长生;Judith K Brown;
Email:
DOI: 10.14083/j.issn.1001-4942.2010.02.019
参考文献(References)：
- [1]Yin QY,Hong Y G,Tian P,et.al.Tomato yellowleaf curl Chi-na virus:monopartite genome organization and agroinfection ofplants[J].Virus Research,2001,81:69-76.
- [2]Xie Y,Zhou X P,Li X G.Molecular characterization of Tomatoyellowleaf curl China virus and its satellite DNA isolated fromtobacco[J].Chinese Sciences Bulletin,2003,48:593-597.
- [3]Wu J B,Dai F M,Zhou X P.First report of tomato yellow leafcurl virus in China[J].Plant Disease,2006,90:1359.
- [4]Mugiira R B,Liu S S,Zhou X.Tomato yellowleaf curl virus andtomato leaf curl Taiwan virus invade Southeast coast of China[J].Journal of Phytopathology,2008,156(4):217-221.
- [5]Wyatt S D,Brown J K.Detection of subgroupⅢgeminivirus i-solates in leaf extracts by degenerate primers and polymerasechain reaction[J].Phytopathology,1996,86(12):1288-1293.
- [6]Ueda S,Takeuchi S,Iwanami T.Evidence of a new tomatoyellow leaf curl virus in Japan and its detection using PCR[J].Genetic Plant Pathology,2005,71:319-325.
- [7]褚栋,周洪旭,王斌,等.山东省烟粉虱与温室白粉虱种群动态及其地理分布调查[J].山东农业科学,2007,2:64-66.
- [8]刘国霞,禇栋,陶云荔.山东省烟粉虱地理分布及部分地区种群动态调查[J].山东农业大学学报,2009,40(2):205-208.
- [9]姜涛,褚栋,姜德锋,等.转Bt+CpTI基因棉对烟粉虱种群动态影响及其几丁质酶和β-1,3-葡聚糖酶活性变化[J].山东农业科学,2009,9:42-47.
- [10]姜涛,褚栋,姜德锋,等.烟粉虱刺吸诱导转Bt+CpTI基因棉苯丙氨酸解氨酶和氧保护酶系活性变化[J].山东农业科学,2009,10:48-53.
- [11]姜涛,姜德锋,褚栋,等.转CrylAc+CpTI双价基因棉对烟粉虱主要解毒酶活性的影响[J].山东农业科学,2008,9:69-72.
- [12]Chu D,Jiang T,Liu G X,et al.Biotype status and distributionofBemisia tabaci(Hemiptera:Aleyrodidae)in Shandong Prov-ince of China based on mitochondrial DNA markers[J].Envi-ronmental Entomology,2007,36:1290-1295.