山东省主产烟区烟草炭疽病菌的鉴定与分子检测Identification and Molecular Detection of Colletotrichum nicotianae Pathogen from Main Tobacco Fields in Shandong Province
贾玉;郑翠梅;张广民;陈秀亭;温亮;田福海;杨永花;
摘要(Abstract):
从山东临沂等地采集烟草炭疽病害标本,并进行分离、检测、鉴定。结果表明,山东烟草炭疽病原为毁灭炭疽菌。根据烟草炭疽菌与其它烟草病害病原菌核糖体转录间隔区(internal transcribed spacerI,TS)序列间的差异,设计了一对特异性引物Co1/Co2。利用此引物可从烟草炭疽菌总DNA中扩增约300 bp的特异性片段,其余菌株和对照PCR结果为阴性。灵敏度实验证明,可以检测到目的片段的DNA浓度为0.1 pg/μl。对土样检测结果表明,该引物能特异性地检测到Colletotrichum destructivum的存在,为快速、灵敏地检测烟草炭疽菌提供了应用依据。
关键词(KeyWords): 烟草炭疽病菌;ITS;PCR;分子检测
基金项目(Foundation): 山东省烟草专卖局科技项目(鲁烟科[2008]20号)
作者(Author): 贾玉;郑翠梅;张广民;陈秀亭;温亮;田福海;杨永花;
Email:
DOI: 10.14083/j.issn.1001-4942.2012.04.013
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